Research on Non-Contact High Voltage Electrostatic Field Microcapsule Preparation Method
2026-04-06 07:51:06··#1
Abstract : To prepare cell microcapsules, a non-contact microcapsule generator based on the principle of high-voltage electrostatic encapsulation was designed and developed. Using sodium alginate in calcium chloride solution for encapsulation, the effects of parameters such as electric field voltage, propulsion speed, electrode distance, and needle type on the particle size of the prepared microcapsules were analyzed, resulting in microcapsules with smooth surfaces, regular shapes, and uniform sizes. Microencapsulated adipose-derived stem cells were prepared using this device. After one week of culture, the cells survived well within the microcapsules and exhibited significant proliferation. Keywords : Non-contact, microcapsule, high-voltage electrostatic, adipose-derived stem cells 0. Introduction Microencapsulation technology originated from the concept of artificial cells proposed by Chang in 1964, which involves encapsulating tissue cells with a biocompatible semi-permeable membrane. Cells within the capsule can obtain small molecule nutrients such as electrolytes and oxygen through the semi-permeable membrane. Small molecule bioactive substances and metabolic products secreted by the cells can be excreted from the microcapsule, while large molecules with immune activity, such as immune cells and immunoglobulins, cannot pass through the capsule membrane. After half a century of development, microencapsulation technology has become a widely used bioengineering technology in the field of cell transplantation medicine. It has played a significant role in the treatment of diabetes, kidney dysfunction, acute liver failure, Parkinson's disease, anemia, and retinal degeneration, and has been listed internationally as a key high-tech area for research and development in the 21st century. [Full text download of research on non-contact high-voltage electrostatic field microcapsule preparation method]